APEX2 (Vector pENTR223.1, Accession No. BC002959)

Length: 1557 Sequence: atgttgcgcg tggtgagctg gaacatcaat gggattcgga gacccctgca aggggtggca aatcaggaac ccagcaactg tgccgccgtg gccgtggggc gcattttgga cgagctggat gcggatatcg tctgtctcca ggaaaccaaa gtgaccaggg atgcactgac agagcccctg gctatcgttg agggttataa ctcctatttc agcttcagcc gcaaccgtag cggctattct ggtgtagcca ccttctgtaa ggacaatgct accccagtgg ctgctgaaga aggcctgagt ggcctgtttg ccacccagaa tggggatgtt ggttgctatg gaaacatgga tgagtttacc caagaggaac tccgggctct ggatagtgag ggcagggccc tcctcacaca gcataagatc cgcacatggg aaggtaagga gaagaccttg accctaatca acgtgtactg cccccatgcg gaccctggga ggcctgagcg gctagtcttt aagatgcgct tctatcgttt gctgcaaatc cgagcagaag ccctcctggc ggcaggcagc catgtgatca ttctgggtga cctgaataca gcccaccgcc ccattgacca ctgggatgca gtcaacctgg aatgctttga agaggaccca gggcgcaagt ggatggacag cttgctcagt aacttggggt gccagtctgc ctctcatgta gggcccttca tcgatagcta ccgctgcttc caaccaaagc aggagggggc cttcacctgc tggtcagcag tcactggcgc ccgccatctc aactatggct cccggcttga ctatgtgctg ggggacagga ccctggtcat agacaccttt caggcctctt tcctgctgcc tgaggtgatg ggctctgacc actgccctgt gggtgcagtc ttgagtgtgt cctctgtgcc tgcaaaacag tgcccacctc tgtgcacccg cttcctccct gagtttgcag gcacccagct caagatcctt cgcttcctag ttcctctcga acaaagtcct gtgttggagc agtcgacgct gcagcacaac aatcaaaccc gggtacagac atgccaaaac aaagcccaag tgcgctcaac caggcctcag cccagtcagg ttggctctag cagaggccag aaaaacctga agagctactt tcagccctcc cctagctgtc cccaagcctc tcctgacata gagctgccta gcctaccact gatgagcgcc ctcatgaccc cgaagactcc agaagagaag gcagtggcca aagtggtgaa ggggcaggcc aagacttcag aagccaaaga tgagaaggag ttacggacct cattctggaa gtctgtgctg gcggggccct tgcgcacacc cctctgtggg ggccacaggg agccatgtgt gatgcgtact gtgaagaagc caggacccaa cttgggccgc cgcttctaca tgtgtgccag gccccggggt cctcccactg acccctcctc ccggtgcaac ttcttcctct ggagcaggcc Protein function: Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'- phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. [The UniProt Consortium]