TOFA

Inhibition of fatty acid synthase (FASN) by the irreversible inhibitor cerulenin leads to cytotoxicity and apoptosis in human cancer cell lines. TOFA is an inhibitor of fatty acid synthesis blocking the synthesis of malonyl-CoA by acetyl-CoA carboxylase (ACC). Both cerulenin (at about 10 µg/ml) and TOFA (at about 1 µg/ml) are effective at blocking the incorporation of radiolabeled acetate into palmitate. However, TOFA reduces malonyl-CoA levels rather than elevating them, and TOFA is relatively non-toxic to various cancer cell lines. TOFA also attenuates the inhibition of feeding observed when FASN inhibitors such as cerulenin and C75 are administered to obese ob/ob mice.Formal Name: 5-(tetradecyloxy)-2-furancarboxylic acid. CAS Number: 54857-86-2. Synonyms: RMI 14514, 5-(Tetradecyloxy)-2-furoic Acid. Molecular Formula: C19H32O4. Formula Weight: 324.5. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMF:PBS (pH 7.2) (1:1): .5 mg/ml, DMSO: 2 mg/ml, Ethanol: 1 mg/ml. lambdamax: 278 nm. SMILES: CCCCCCCCCCCCCCOc1ccc(o1)C(=O)O. InChi Code: InChI=1S/C19H32O4/c1-2-3-4-5-6-7-8-9-10-11-12-13-16-22-18-15-14-17(23-18)19(20)21/h14-15H,2-13,16H2,1H3,(H,20,21). InChi Key: CZRCFAOMWRAFIC-UHFFFAOYSA-N.