Arachidonoyl m-Nitroaniline

Arachidonoyl m-Nitroaniline (AmNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity. FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide (arachidonyl ethanolamide, AEA). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate (see also Decanoyl m-Nitroaniline, Cay-90349). Exposure of AmNA to FAAH activity results in the release of the yellow colorimetric dye m-nitroaniline (epsilon = 13,500 at 410 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96 well plate spectrophotometer.Formal Name: N-(3-nitrophenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide. CAS Number: 1175954-87-6. Synonyms: AmNA. Molecular Formula: C26H36N2O3. Formula Weight: 424.6. Purity: >98%. Formulation: (Request formulation change), A solution in methyl acetate. Solubility: DMF: 50 mg/ml, DMSO: 50 mg/ml, DMSO:PBS (1:4): 1 mg/ml, Ethanol: 50 mg/ml. lambdamax: 203, 242 nm. SMILES: CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)Nc1cccc(c1)[N](=O)O. InChi Code: InChI=1S/C26H36N2O3/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-22-26(29)27-24-20-19-21-25(23-24)28(30)31/h6-7,9-10,12-13,15-16,19-21,23H,2-5,8,11,14,17-18,22H2,1H3,(H,27,29)/b7-6-,10-9-,13-12-,16-15-. InChi Key: OITDPRAMDXERDT-DOFZRALJSA-N.