XFD700 Maleimide

XFD700, manufactured by AAT Bioquest, is a near-infrared fluorescent dye structurally similar to Alexa Fluor(TM) 700 (Thermo Fisher). It is optimally excited by 633-640 nm laser lines and offers a relatively low fluorescence intensity, making it particularly well-suited for direct imaging of high-abundance targets in both microscopy and flow cytometry. This allows researchers to allocate brighter dyes for detecting lower-abundance antigens, improving overall panel design. XFD700 exhibits excellent aqueous solubility and maintains consistent fluorescence stability across a broad pH range (pH 4-10), ensuring robust and reproducible performance under diverse experimental conditions. Its long-wavelength emission effectively minimizes background autofluorescence, leading to enhanced signal-to-noise ratios, especially in complex biological samples such as tissues. In multicolor flow cytometry panels, XFD700 serves as an ideal option between APC and APC-iFluor(R) 780, enabling better resolution in complex assays. The maleimide derivative of XFD700 is widely used for labeling biomolecules with free thiol (SH) groups, including antibodies, proteins, thiol-modified oligonucleotides, and low molecular weight ligands. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications.