XFD568 C5 maleimide

XFD568, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor(TM) 568 (Thermo Fisher). This bright orange-fluorescent dye is efficiently excited by the 568 nm line of the AR-Kr mixed-gas laser and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4-10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD568 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced imaging techniques like dSTORM. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD568 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays. The maleimide derivative of XFD568 is widely used for labeling biomolecules with free thiol (SH) groups, including antibodies, proteins, thiol-modified oligonucleotides, and low molecular weight ligands. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications.