XFD546 TCO

Product key features: Ex/Em:561/572 nm. Extinction coefficient: 112000 cm-1M-1. Correction factor (260 nm): 0.21. Correction factor (280 nm): 0.12. Quantum yield: 0.79. Reactive Group: TCO Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules Consistent Fluorescence: pH-insensitive from pH 4 to 10 and minimal quenching when conjugated to proteins High Photostability: Ensures reliable performance for multicolor flow cytometry and advanced imaging applications like STORM. Product description: XFD546, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor(TM) 546 (ThermoFisher). XFD546 TCO is a tetrazine-reactive, orange fluorescent dye with an excitation/emission of 561/572 nm that is used for flow cytometry and microscopy applications. Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules High Quantum Yield & Stability: Exhibits intense fluorescence with resilience to photobleaching, thermal fluctuations, and pH variations from 4 to 10 Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies. XFD546, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor(TM) 546 (Thermo Fisher). It exhibits bright orange fluorescence and is readily excited by laser lines at 488 nm or 532 nm, making it highly suitable for applications such as fluorescence microscopy and flow cytometry. XFD546 demonstrates excellent aqueous solubility and pH-insensitivity over a broad range (pH 4-10), ensuring stable signal generation under varying experimental conditions. Additionally, the dye enables high molar ratio conjugation to proteins with minimal fluorescence quenching, facilitating the generation of brighter conjugates for enhanced detection sensitivity. With its high fluorescence quantum yield and superior photostability, XFD546 is particularly advantageous for detecting low-abundance biological targets, providing researchers with improved sensitivity and precision in quantitative fluorescence-based assays. XFD546 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.