XFD514 maleimide

XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally similar to Alexa Fluor(TM) 514 (Thermo Fisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4-10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays. The maleimide derivative of XFD514 is widely used for labeling biomolecules with free thiol (SH) groups, including antibodies, proteins, thiol-modified oligonucleotides, and low molecular weight ligands. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications.