ReadiUse(TM) Preactivated PE-iFluor(TM) 700 Tandem

PE-Alexa Fluor(R) 700 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 720 nm. AAT Bioquest offers this preactivated PE-iFluor 700(TM) as a superior replacement to the popular PE- Alexa Fluor(R) 700 tandem. Compared to PE-Alexa Fluor(R) 700 tandem, PE-iFluor 700 is brighter with better FRET efficiency. It is used to facilitate the PE-Alexa Fluor(R) 700 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-iFluor 700 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based PE-Alexa Fluor(R) 700 tandem conjugation chemistry with better flow cytometry performance. In addition, our preactivated PE-iFluor 700 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.