Protonex(TM) Red 600

Ex: 575 nm. Em: 597 nm. Soluble in: DMSO. ProtonexT Red dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of ProtonexT Red dye. The fluorescence of ProtonexT Red dye dramatically increases as pH decreases from neutral to the acidic. The lack of fluorescence outside the cell eliminates the wash steps. ProtonexT Red dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. ProtonexT Red dye is non-fluorescent outside the cells, but fluoresces brightly red in acidic compartments (such as phagosomes, lysosomes and endosomes). This ProtonexT Red enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. It can be also used for multiplexing cellular functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. ProtonexT Red has the spectral properties similar to those of Texas Red, making the common filter set of Texas Red readily available to the assays of ProtonexT Red.