Phen Green(TM) SK, Diacetate

Phen Green(TM) SK, Diacetate is a cell-permeable, fluorogenic chelator designed for the sensitive detection of intracellular transition and heavy metal ions in live-cell applications. Upon cellular uptake, cytosolic esterases efficiently hydrolyze the diacetate protecting groups, yielding the highly fluorescent, metal-sensitive Phen Green(TM) SK dye. The activated probe exhibits excitation and emission maxima at approximately 507 nm and 532 nm, respectively, and undergoes fluorescence quenching in the presence of a broad range of divalent and monovalent metal ions, including Fe2+, Cu2+, Cu+, Zn2+, Cd2+, Ni2+, Co2+, Hg2+, and Pb2+. This metal-induced quenching mechanism allows for direct, concentration-dependent monitoring of labile metal pools within the cytosol. Phen Green(TM) SK, Diacetate is well-suited for use in both fluorescence microscopy and high-throughput microplate assays, offering flexibility across experimental platforms. Its broad metal ion reactivity and robust signal response make it a powerful tool for investigating intracellular metal ion dynamics, redox regulation, metal transport mechanisms, and metal-associated cytotoxicity in diverse biological systems.