OxiVision(TM) Red Lipid Peroxidation Sensor

The OxiVision(TM) Red Lipid Peroxide Sensor is a perylene-based fluorescent probe functionalized with oligooxyethylene for the selective detection of lipid peroxides. Upon oxidation by lipid peroxides, the sensor emits intense fluorescence in organic solvents such as ethanol. Unlike conventional ROS probes, OxiVision(TM) Red demonstrates a high degree of selectivity for lipid peroxides, enabling precise monitoring of lipid peroxidation dynamics. The oxidized form of the probe exhibits excitation and emission maxima at 650 nm and 670 nm, respectively, optimizing detection by reducing phototoxicity and minimizing autofluorescence in biological samples. The addition of a tetraethyleneglycol moiety on the diisoquinoline ring improves solubility and dispersibility in aqueous environments, enhancing probe stability and usability in biological assays. Notably, the oxidized probe exhibits negligible fluorescence in aqueous media but displays a pronounced increase in fluorescence intensity in lipophilic environments, such as cellular membranes. This fluorescence shift enables quantitative assessment of lipid peroxides in live-cell systems using flow cytometry and facilitates high-resolution imaging of lipid peroxidation via fluorescence microscopy. The probe provides a robust tool for investigating oxidative lipid modifications, particularly in studies related to ferroptosis and lipid peroxidation-mediated cell death pathways.