Nuclear Blue(TM) DCS2 *Equivalent to SYTOX(TM) Blue Dead Cell Stain*

Nuclear Blue(TM) DCS2 is the same molecule to SYTOX(TM) Blue Dead Cell Stain (SYTOX(TM) is a trademark of ThermoFisher). Nuclear Blue(TM) DCS2 is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. It is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes. Under the same conditions, our Nuclear Violet(TM) DCS1 (#17549) gives much higher signal/background ratio than SYTOX(TM) Blue Dead Cell Stain. Nuclear Violet(TM) DCS1 is better excited by the violet laser at 405 nm than SYTOX(TM) Blue Dead Cell Stain. After brief incubation with Nuclear Violet(TM) DCS1 stain, the nucleic acids of dead cells fluoresce bright blue when excited with 405 nm violet laser light. The violet-excited fluorescence emission of Nuclear Violet(TM) DCS1 stain permits clear discrimination from probes excited by most other laser lines, facilitating the development of multicolor assays with minimal spectral overlap between signals. Solvent: DMSO. Flow cytometer: Excitation: 405 nm Laser, Emission: 473/15 nm Filter. Excitation (nm): 445. Emission (nm): 470.