mFluor(TM) Violet 500 SE

AAT Bioquest's mFluor(TM) dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor(TM) Violet 500 dyes have fluorescence excitation and emission maxima of ~405 nm and ~500 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Green(TM) (ThermoFisher) and BD Horizon(TM) V500 labeling dyes. mFluor(TM) Violet 500 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor(TM) Violet 500 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. Under the same conditions, mFluor(TM) Violet 500 dye conjugates are significantly brighter than the corresponding bioconjugates of Pacific Green(TM) (ThermoFisher) and BD Horizon(TM) V500 with much stronger absorption, making the mFluor(TM) Violet 500 conjugates much more sensitive.