Lucigenin

Lucigenin is a chemiluminescent probe used to detect superoxide production and the presence of chloride. It can be used to detect superoxide production by enzymatic and cellular sources. It is a sensitive method that has been applied to the monitoring of superoxide production from xanthine oxidase, microsomal NADPH cytochrome reductase, and NADPH oxidases of phagocytes, endothelial cells, fibroblasts, and smooth muscle cells of blood vessel walls. However, it produces similar chemiluminescence signals in isolated aortic and cardiac tissues from wild-type and Nox1-Nox2-Nox4 triple knockout mice, suggesting that it is not selective for NADPH-based ROS production. It also reacts with hydrogen peroxide without generating free radical intermediates and has been used to detect lipid hydroperoxide in oils. Lucigenin is also used as a fluorescent chloride-sensitive indicator, with its fluorescence being quenched by chloride (ex/em = 455/505 nm, respectively). Lucigenin fluorescence is insensitive to phosphate, sulfate, and nitrate.Formal Name: 10,10'-dimethyl-9,9'-biacridinium, dinitrate. CAS Number: 2315-97-1. Synonyms: L-6,868, NSC 151912. Molecular Formula: C28H22N2 . 2NO3. Formula Weight: 510.5. Purity: >95%. Emission: 505 nm. Excitation: 455 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 0.33 mg/ml, DMSO: 5 mg/ml, PBS (pH 7.2): 1 mg/ml. lambdamax: 222, 261, 369, 429 nm. SMILES: C[N+](C1=C2C=CC=C1)=C3C(C=CC=C3)=C2C4=C5C(C=CC=C5)=[N+](C)C6=CC=CC=C64.O=[N+]([O-])[O-].O=[N+]([O-])[O-]. InChi Code: InChI=1S/C28H22N2.2NO3/c1-29-23-15-7-3-11-19(23)27(20-12-4-8-16-24(20)29)28-21-13-5-9-17-25(21)30(2)26-18-10-6-14-22(26)28,2*2-1(3)4/h3-18H,1-2H3,,/q+2,2*-1. InChi Key: KNJDBYZZKAZQNG-UHFFFAOYSA-N.