iFluor(TM) 750 succinimidyl ester

Molecular weight: 1416.83 Excitation (nm): 757, Emission (nm): 779, Extinction coefficient (cm-1 M-1): 275000, Quantum yield: 0.12, Correction factor (280 nm): 0.039. AAT Bioquest's iFluorT dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluorT 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with good photostability. Our in-house comparable studies indicated that our iFluorT 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen respectively). iFluorT 750 conjugates have been widely used in fluorescence animal imaging applications. The iFluorT 750 SE dye is reasonably stable and shows good reactivity and selectivity with proteins.