iFluor(TM) 594 TCO

Molecular weight: 1045.11. Excitation (nm): 588. Emission (nm): 604. Extinction coefficient (cm-1 M-1): 180000. Quantum yield: 0.53. Correction factor (280 nm): 0.04. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor(TM) 594-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor(TM) 594 dye have spectral properties almost identical to the popular Cy3.5 and Alexa Fluor(R) 594. In the most cases, the antibody conjugates prepared with iFluor(TM) 594 tend to have lower background than the spectrally similar dye conjugates (sch as Cy3.5 and Alexa Fluor(R) 594, Alexa Fluor(R) is the trademark of Invitrogen).