iFluor(TM) 440 succinimidyl ester

Molecular weight: 692.83. Excitation (nm): 434. Emission (nm): 480. Absorbance (nm): 430. Extinction coefficient (cm-1 M-1): 40000. Correction factor (280 nm): 0.229. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(TM) 440 dyes are designed to be a superior replacement for DEAC dye that has extremely poor water solubility. iFluor(TM) 440 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. Under the same conditions, iFluor(TM) 440 dye conjugates are significantly brighter than the corresponding bioconjugates of DEAC or other spectrally similar dyes (such as SpectrumAqua), making the iFluor 440 conjugates much more sensitive.