iFluor(R) Ultra 647 maleimide

The iFluor(R) Ultra series represents an enhancement of our established iFluor(R) dyes, optimized for antibody labeling in fluorescence imaging and flow cytometry. Within this series, iFluor(R) Ultra 647 is an exceptionally bright far-red fluorescent dye excitable by 594 nm or 633 nm laser lines, with a peak emission at 670 nm. Antibody conjugates with iFluor(R) Ultra 647 exhibit superior brightness compared to those labeled with Alexa Fluor(R) 647 under identical conditions. Additionally, iFluor(R) Ultra 647 maintains stable fluorescence across a pH range of 4 to 10. The maleimide derivative of iFluor(R) Ultra 647 is widely used for conjugation to thiol groups on proteins, oligonucleotide thiophosphates, or low molecular weight ligands. Conjugates with iFluor(R) Ultra 647 demonstrate higher fluorescence intensity and greater photostability compared to those with other spectrally similar fluorophores, enhancing their utility for advanced fluorescence applications. Fluorescent dye-conjugated antibodies are crucial for protein identification in various applications, including fluorescent cell imaging, flow cytometry, western blotting, and immunohistochemistry. The advantages of these conjugates include increased sensitivity, multiplexing capabilities, and ease of use, facilitating complex biological studies.