Glucose-UDP-(PEG)6-Fluorescein Conjugate

Uridine-5'-diphospho-1-alpha-D-glucose (UDP-Glc) is a key intermediate in carbohydrate metabolism. UDP-Glc serves as a precursor of glycogen, can be metabolized into UDP-galactose and UDP-glucuronic acid which can then be incorporated into polysaccharides as galactose and glucuronic acid. UDP-Glc serves as a precursor of sucrose lipopolysaccharides and glycosphingolipids. UDP-Glc is a common substrate used by glucosyltransferases, including certain bacterial toxins such as Toxins A and B from Clostridium dicile. Fluorescent analogs of UDP-Glc might be used for studying glucosyltransferases and clostridial toxins. This fluorescein UDGlc derivative has the spectral properties identical to those of FITC conjugates. Compared to ABD-11705, this UDP-Glc derivative has a much longer spacer between the fluorophore and UDP moiety.