DPA

Dipicrylamine (DPA) is a nonfluorescent anionic membrane dye. With its absorbance in the blue region of the visible spectrum, DPA can be used as a FRET quencher for a variety of fluorescent donor dyes. When applied to cells, DPA localizes at the interface of cytoplasmic membrane and aqueous phase in a manner sensitive to membrane potential change. A recent application of DPA is to measure cell membrane potential change by combining with the fluorescent donor dye DiOC16. The DiO/DPA system has been reported to produce a fluorescence signal change of >,56% in HEK-293 cells and >,25% in neuronal cultures and brain slices per 100 mV membrane potential change. Please also see the DiO/DPA Membrane Potential Detection Kit. Yellow solid soluble in DMSO at >,30mg/mL&lamba,abs (MeOH) = 406 nm,Store at 4°C,MWt: 439.21,[131-73-7],