Cal-520(R) amine

Excitation (nm): 493 Emission (nm): 515 Quantum yield: 0.75 (calcium bound (pH 7.2)). Cal-520(R) amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to a carboxy group via activated esters or EDC coupling chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520(R) has been proven to be one of the best-known green fluorescent calcium indicators. Solvent: DMSO.