Biotin-(5-fluorescein)-conjugate

Synthetic. Chemical. CAS: 957494-27-8. Formula: C33H32N4O8S. MW: 644.7. Synthetic. Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm, lambdaem 524nm in 0.1M Tris pH8.0.