APC-iFluor(TM) 700 Tandem

Excitation (nm): 651. Emission (nm): 710. Extinction coefficient (cm-1 M-1): 700000. Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Its strong absorption at 633 nm and 647 nm makes the tandem one of the best fluorophores used with a red laser in flow cytometry applications. APC-XFD 700 has the same structure to Alexa Fluor(R) 700 tandem. It is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~710 nm.