ABD-F

White to yellow powder. Soluble in 1M ammonium hydroxide (50 mg/ml). Highly reactive fluorogenic reagent for the labeling of thiols for HPLC. ABD-F is nonfluorescent until reacted with thiols and therefore can be used to quantitate thiols in solution, as well as thiols separated by HPLC or TLC. ABD-F also reportedly reacts slowly with the hydroxy group of some tyrosine residues as well as amino groups in some proteins, forming products that are nonfluorescent but can be detected by absorbance at 385 nm.