6-ROXtra(TM) SE *Superior 6-ROX Replacement*

Molecular weight: 1030.23. Excitation (nm): 578. Emission (nm): 595. Although ROX dyes have strong fluorescence they are notoriously unstable. Some commercial samples only have a few months' shelf life. Compared to 6-ROX labeling compounds our 6-ROXtra(TM) dyes have greatly enhanced stability while they have essentially identical spectral properties to those of 6-ROX when conjugated to a biological substrate (such as oligonucleotides). In addition, our 6-ROXtra(TM) dyes are much more water-soluble than 6-ROX dyes that are essentially insoluble in water and aqueous buffers. Our preliminary studies indicated that 6-ROXtra-derived oligos are more easily purified than the corresponding 6-ROX oligos in most cases. When conjugated to proteins, the fluorescence of 6-ROX is severely quenched while 6-ROXtra(TM) still shows strong fluorescence.