5-Carboxyfluorescein

5-Carboxyfluorescein is a single isomer derivative of 5(6)-carboxyfluorescein (Cay-17172) that can be used to fluorescently label biomolecules through the interaction of carboxylic acid with primary amines. It demonstrates excitation/emission maxima of 492 and 518 nm, respectively.Formal Name: 3',6'-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid. CAS Number: 76823-03-5. Synonyms: 5-FAM. Molecular Formula: C21H12O7. Formula Weight: 376.3. Purity: >98%. Emission: 518 nm. Excitation: 492 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: 0.5 mg/ml, Ethanol: 5 mg/ml, Ethanol:PBS (pH 7.2) (1:1): 0.5 mg/ml. lambdamax: 209, 225, 277 nm. SMILES: OC1=CC=C(C2(C(C=CC(C(O)=O)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1. InChi Code: InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25). InChi Key: NJYVEMPWNAYQQN-UHFFFAOYSA-N.