XFD647-UTP

XFD647, manufactured by AAT Bioquest, is a far-red fluorescent dye that is structurally similar to Alexa Fluor(TM) 647 (Thermo Fisher), offering high photostability and intense fluorescence. When conjugated to uridine triphosphate (UTP), XFD647-UTP functions as an alternative to natural UTP in T7 RNA polymerase-mediated in vitro transcription, enabling the synthesis of fluorescently labeled RNA probes. These probes are highly suited for multicolor fluorescence-based applications, including dual-color expression profiling, microarrays, fluorescence in situ hybridization (FISH), chromosome identification, whole chromosome painting, karyotyping, and gene mapping. XFD647-UTP efficiently incorporates into RNA transcripts through T7 RNA polymerase, imparting robust fluorescence for the visualization and quantification of RNA localization, distribution, and dynamics in live or fixed-cell systems. With excitation and emission maxima at 650 nm and 671 nm, respectively, XFD647-UTP-labeled RNA is readily detectable under fluorescence microscopy and gel electrophoresis without additional post-staining. Furthermore, XFD647-UTP facilitates single-molecule fluorescence resonance energy transfer (smFRET) experiments, allowing real-time monitoring of co-transcriptional RNA folding and structural dynamics at the molecular level. The exceptional photostability and narrow emission profile of XFD647-labeled nucleotides make them highly advantageous for multiplexed fluorescence imaging, enabling precise signal discrimination in complex biological samples.