Tris Glycine SDS Transfer Buffer 10X

Dilute 100ml Tris Glycine Transfer Buffer 10X with 900 ml deionised water to make 1 litre of Tris Glycine Buffer. Final concentration is 25 mM Tris Base 0.192 M Glycine and 1g/l SDS. pH, 20 C. 8.45 ± 0.01 at 1 x use rate Final concentration after diluting   TRIS 0.25 M 30.3 g/L Glycine 1.92 M 144.1 g/L SDS 10.0 g/L. Store at room temperature. Keep away from light.