SPQ

SPQ is a fluorescent chloride indicator. Its fluorescence is rapidly quenched by chloride in vitro. It has been used to assess voltage-sensitive chloride transport in placental microvillus vesicles. It has also been used to continuously quantify intracellular calcium efflux induced by parathyroid hormone, forskolin (Cay-11018), and phorbol 12-myristate 13-acetate (PMA, Cay-10008014) in isolated human erythrocytes. In addition, SPQ has been used to determine the type of ion conductance induced by the cystic fibrosis transmembrane conductance regulator (CFTR) in HEK293 cells expressing pTrial10-CRTR2. SPQ displays excitation maxima of 320 and 350 nm and an emission maximum of 445 nm.Formal Name: 6-methoxy-1-(3-sulfopropyl)-quinolinium, inner salt. CAS Number: 83907-40-8. Molecular Formula: C13H15NO4S. Formula Weight: 281.3. Purity: >95%. Emission: 445 nm. Excitation: 320 and 350 nm. Formulation: (Request formulation change), A solid. Solubility: Water: soluble. SMILES: COC1=CC(C=CC=[N+]2CCCS([O-])(=O)=O)=C2C=C1. InChi Code: InChI=1S/C13H15NO4S/c1-18-12-5-6-13-11(10-12)4-2-7-14(13)8-3-9-19(15,16)17/h2,4-7,10H,3,8-9H2,1H3. InChi Key: ZVDGOJFPFMINBM-UHFFFAOYSA-N.