Phosphine-biotin

Phosphine biotin is a labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction. Modified proteins can be detected using common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE. For example, phosphine-biotin has been used successfully in conjunction with DAz-1 or DAz-2 to label and detect sulfenic acid sites in proteins.Formal Name: 2-(diphenylphosphino)-4-[21-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1,17-dioxo-6,9,12-trioxa-2,16-diazaheneicos-1-yl]-benzoic acid, methyl ester. CAS Number: 608514-42-7. Synonyms: Click Tag(TM) Phosphine-biotin. Molecular Formula: C41H53N4O8PS. Formula Weight: 792.9. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 2 mg/ml. SMILES: O=C1N[C@]2([H])[C@]([C@H](CCCCC(NCCCOCCOCCOCCCNC(C3=CC=C(C(OC)=O)C(P(C4=CC=CC=C4)C5=CC=CC=C5)=C3)=O)=O)SC2)([H])N1. InChi Code: InChI=1S/C41H53N4O8PS/c1-50-40(48)33-19-18-30(28-35(33)54(31-12-4-2-5-13-31)32-14-6-3-7-15-32)39(47)43-21-11-23-52-25-27-53-26-24-51-22-10-20-42-37(46)17-9-8-16-36-38-34(29-55-36)44-41(49)45-38/h2-7,12-15,18-19,28,34,36,38H,8-11,16-17,20-27,29H2,1H3,. InChi Key: ABJATKZPSPFIQY-CELQPYBTSA-N.