Nile Red

Nile red is a fluorescent probe commonly used for the detection of intracellular lipid droplets. It displays excitation/emission maxima of 529/576 nm, respectively, for neutral lipids. It is selective for neutral lipids at emission wavelengths of 580 nm or lower, but, at higher emission wavelengths, phospholipid membranes are also visualized. Nile red has been used in live, fixed, or unfixed cells with detection by fluorescence microscopy, spectroscopy, or flow cytometry. It has been used as a marker of lipid droplets in cellular models of hepatocyte steatosis.Formal Name: 9-(diethylamino)-5H-benzo[a]phenoxazin-5-one. CAS Number: 7385-67-3. Molecular Formula: C20H18N2O2. Formula Weight: 318.4. Purity: >95%. Emission: 576 nm, for neutral lipids. Excitation: 529 nm, for neutral lipids. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: 1 mg/ml, DMSO:PBS (pH 7.2) (1:10): 0.09 mg/ml, Ethanol: slightly soluble. lambdamax: 265, 553 nm. SMILES: O=C1C=C2OC3=CC(N(CC)CC)=CC=C3N=C2C4=CC=CC=C14. InChi Code: InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3. InChi Key: VOFUROIFQGPCGE-UHFFFAOYSA-N.