Neferine

Neferine is an alkaloid that has been found in N. nucifera and has diverse biological activities. It inhibits ADP-, collagen-, thrombin-, U46619-, or adrenaline-induced aggregation of isolated human platelets when used at a concentration of 3 µM. Neferine (2 µM) prevents LPS- and ATP-induced pyroptosis, NLRP3 inflammasome activation, and the production of reactive oxygen species (ROS) in human umbilical vein endothelial cells (HUVECs). It decreases expression of the genes encoding peroxisome proliferator-activated receptor gamma (PPARgamma), CCAAT/enhancer-binding protein alpha (C/EBPalpha), sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (FASN) and reduces lipid accumulation in 3T3-L1 cells. Neferine enhances TRAIL-induced LC3-II accumulation, a marker of autophagy, and cell death in DU145 prostate cancer cells. In vivo, neferine (25 and 50 mg/kg) improves reference and working memory, as well as reduces hippocampal lipid peroxidation and IL-6, IL-1beta, and TNF-alpha levels, in a rat model of aluminum chloride-induced Alzheimer's disease.Formal Name: 4-[[(1S)-1,2,3,4-tetrahydro-6,7-dimethoxy-2-methyl-1-isoquinolinyl]methyl]-2-[[(1R)-1,2,3,4-tetrahydro-6-methoxy-1-[(4-methoxyphenyl)methyl]-2-methyl-7-isoquinolinyl]oxy]-phenol. CAS Number: 2292-16-2. Synonyms: 4''-O-Methylliensinine, (-)-Neferine. Molecular Formula: C38H44N2O6. Formula Weight: 624.8. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMF:PBS (pH 7.2) (1:5): 0.16 mg/ml, DMSO: 20 mg/ml, Ethanol: 10 mg/ml, PBS (pH 7.2): isnoluble. SMILES: COC1=CC=C(C[C@H]2N(CCC3=CC(OC)=C(OC4=CC(C[C@@H]5N(CCC6=CC(OC)=C(OC)C=C65)C)=CC=C4O)C=C32)C)C=C1. InChi Code: InChI=1S/C38H44N2O6/c1-39-15-14-27-21-36(44-5)38(23-30(27)31(39)17-24-7-10-28(42-3)11-8-24)46-34-19-25(9-12-33(34)41)18-32-29-22-37(45-6)35(43-4)20-26(29)13-16-40(32)2/h7-12,19-23,31-32,41H,13-18H2,1-6H3/t31-,32+/m1/s1. InChi Key: MIBATSHDJRIUJK-ZWXJPIIXSA-N. Origin: Plant/Plumula Nelumbinis.