MOPS - SDS Running Buffer 20X

MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins and is suitable for protein gel electrophoresis. The use of MOPS Buffer allows proteins to run slower than when using MES buffer. Formedium recommend diluting 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer. This makes a final concentration of 50mM MOPS, 50mM TRIS base, 3.47mM SDS and 1mM EDTA. This buffer is used in 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis. Specifications   MOPS 1  M  209.3  g/L TRIS 1  M  121.1  g/L SDS 69.3  mM  20  g/L EDTA Na2 20.5  mM  7.6  g/L Store at room temperature Keep away from light