L-trans-Pyrrolidine-2,4-dicarboxylic Acid

L-trans-Pyrrolidine-2,4-dicarboxylic acid (L-trans-2,4-PDC) is an inhibitor of L-glutamate transport (Ki = 4.6 µM in rat brain synaptosomes). It inhibits radioligand binding to NMDA receptors by 13% but has no effect on AMPA or kainate receptors when used at a concentration of 100 µM. L-trans-2,4-PDC is neurotoxic to astrocyte-rich and astrocyte-poor rat cortical cultures (EC50s = 320 and 50 µM, respectively), an effect that can be reversed by the NMDA antagonist MK-801 (Cay-10009019) and glutamate-pyruvate transaminase but not the non-NMDA glutamate receptor antagonist CNQX (Cay-14618). It induces efflux of the non-metabolizable glutamate analog [3H]-D-aspartate in an extracellular sodium-dependent manner. In vivo, L-trans-2,4-PDC (0.05-0.2 µg/side) prevents amphetamine-induced hyperlocomotion in a dose-dependent manner in rats when injected directly into the nucleus accumbens. It also increases bladder intercontraction interval (ICI) without affecting postvoid residual or basal pressure in rats.Formal Name: 2S,4R-pyrrolidinedicarboxylic acid. CAS Number: 64769-66-0. Synonyms: L-trans-2,4-PDC. Molecular Formula: C6H9NO4. Formula Weight: 159.1. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: PBS (pH 7.2): 2 mg/ml. SMILES: OC([C@H]1NC[C@H](C(O)=O)C1)=O. InChi Code: InChI=1S/C6H9NO4/c8-5(9)3-1-4(6(10)11)7-2-3/h3-4,7H,1-2H2,(H,8,9)(H,10,11)/t3-,4+/m1/s1. InChi Key: NRSBQSJHFYZIPH-DMTCNVIQSA-N.