L-Leucine-7-amido-4-methylcoumarin hydrochloride

Description: L-Leu-AMC is a fluorogenic substrate for leucine aminopeptidase.1Upon enzymatic cleavage by leucine aminopeptidase, AMC is released and its fluorescence can be used to quantify leucine aminopeptidase activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. Smiles: Cl.CC(C)C[C@H](N)C(=O)Nc1ccc2c(C)cc(=O)oc2c1. References: Izquierdo, M., Lin, D., O'Neill, S., et al.Development of a high-throughput screening assay to identify inhibitors of the major M17-leucyl aminopeptidase from Trypanosoma cruzi using rapidfire mass spectrometrySLAS Discov.25(9)1064-1071(2020)