Hispidin

Hispidin is a polyphenol originally isolated from P. hispidus that has diverse biological activities, including antioxidant, anti-inflammatory, and cytoprotective properties. In a trolox equivalent antioxidant capacity (TEAC) assay, hispidin scavenges radicals at 14.47 equivalents of trolox (Cay-10011659). It inhibits transcriptional activity of NF-kappaB, decreases inducible nitric oxide synthase (iNOS) expression, and decreases the generation of reactive oxygen species (ROS) in LPS-induced macrophage RAW 264.7 cells. Hispidin inhibits apoptosis and increases insulin secretion in hydrogen peroxide-treated RINm5F pancreatic beta-cells. It inhibits protein kinase C beta (PKCbeta, IC50 = 2 µM) with no activity against alkaline phosphatase. Hispidin also inhibits beta-secretase (BACE1, IC50 = 4.9 µM) and prolyl endopeptidase (PE, IC50 = 16 µM) but not other serine proteases when used at a concentration of 40 µM (0.6, 0, 8.2, and 3.1% inhibition of chymotrypsin, trypsin, elastase, and tumor necrosis factor-alpha converting enzyme (TACE), respectively).Formal Name: 6-[(1E)-2-(3,4-dihydroxyphenyl)ethenyl]-4-hydroxy-2H-pyran-2-one. CAS Number: 555-55-5. Molecular Formula: C13H10O5. Formula Weight: 246.2. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: >10 mg/ml. lambdamax: 221, 255, 379 nm. SMILES: OC1=CC(/C=C/C(O2)=CC(O)=CC2=O)=CC=C1O. InChi Code: InChI=1S/C13H10O5/c14-9-6-10(18-13(17)7-9)3-1-8-2-4-11(15)12(16)5-8/h1-7,14-16H/b3-1+. InChi Key: SGJNQVTUYXCBKH-HNQUOIGGSA-N.