Fluo-4, Pentapotassium Salt

Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications. Ex: 494 nm. Em: 516 nm.