Ethidium (bromide)

Ethidium is a phenanthridine fluorescent dye and DNA intercalator. It displays an emission at 595 nm upon excitation at 525 nm. Ethidium inhibits the DNA-unwinding and ATPase activities of P. falciparum DNA helicase (IC50s = 1.5 and 2.5 µM, respectively) and is active against P. falciparum in vitro. It completely inhibits E. coli RNA polymerase when used at a concentration of 100 µmol/ml and is active against Mycoplasma when used at concentrations ranging from 0.5 to 8 µg/ml. Ethidium (8 mg/kg) increases lifespan in 6C3HED-OG and L5178Y murine lymphoma models. It has commonly been used to stain DNA in agarose gel electrophoresis and in displacement assays to determine compound binding to DNA.Formal Name: 3,8-diamino-5-ethyl-6-phenyl-phenanthridinium, monobromide. CAS Number: 1239-45-8. Synonyms: Dromilac, Homidium Bromide. Molecular Formula: C21H20N3 . Br. Formula Weight: 394.3. Purity: >95%. Emission: 595 nm. Excitation: 525 nm. Formulation: (Request formulation change), A solid. Solubility: DMSO: 10 mg/ml. SMILES: CC[N+]1=C(C=C2N)C(C=C2)=C(C=C3)C(C=C3N)=C1C4=CC=CC=C4.[Br-]. InChi Code: InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14,/h3-13,23H,2,22H2,1H3,1H. InChi Key: ZMMJGEGLRURXTF-UHFFFAOYSA-N.