DC_AC50

DC_AC50 is an inhibitor of the copper-trafficking proteins Atox1 and CCS. It binds to purified Atox1 and CCS (Kds = 6.8 and 8.2 µM, respectively) and inhibits the interaction between Atox1 and domain 4 of the Cu+-ATPase ATP7B in the presence of zinc in a FRET-based assay when used at a concentration of 100 µM. DC_AC50 increases copper and reactive oxygen species (ROS) levels and decreases COX activity and lipid biosynthesis in H1299 cells when used at a concentration of 10 µM. It inhibits the proliferation of H1299, K562, MDA-MB-231, and 212LN cancer cells, but not PIG1, HDF, HaCaT, or MCF-10A cells, in a concentration-dependent manner. DC_AC50 (100 mg/kg per day) reduces tumor growth in an H1299 mouse xenograft model.Formal Name: 3-amino-N-(2-bromo-4,6-difluorophenyl)-6,7-dihydro-5H-cyclopenta[b]thieno[3,2-e]pyridine-2-carboxamide. CAS Number: 497061-48-0. Molecular Formula: C17H12BrF2N3OS. Formula Weight: 424.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 10 mg/ml. lambdamax: 294 nm. SMILES: NC1=C(C(NC2=C(F)C=C(F)C=C2Br)=O)SC3=NC4=C(CCC4)C=C31. InChi Code: InChI=1S/C17H12BrF2N3OS/c18-10-5-8(19)6-11(20)14(10)23-16(24)15-13(21)9-4-7-2-1-3-12(7)22-17(9)25-15/h4-6H,1-3,21H2,(H,23,24). InChi Key: DFNOJNBNTVQPCA-UHFFFAOYSA-N.