4beta-Hydroxywithanolide E

4beta-Hydroxywithanolide E is a withanolide steroidal lactone that has been found in P. peruviana and has anti-inflammatory and anticancer activities. It inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and TNF-alpha-induced NF-kappaB activity in HEK293 cells (IC50s = 0.32 and 0.04 µM, respectively). 4beta-Hydroxywithanolide E (5 µM) inhibits LPS-induced increases in inducible nitric oxide synthase (iNOS) and COX-2 levels and Akt and STAT1 phosphorylation in RAW 264.7 cells. It inhibits Wnt signaling in HCT116 and SW480 colorectal cancer cells (IC50s = 1.85 and 2.67 µM, respectively). 4beta-Hydroxywithanolide E inhibits the proliferation of HCT116, SW480, HT-29, and LoVo cells (IC50s = 0.24-0.51 µM). It halts the cell cycle at the G0/G1 phase in HCT116 and HT-29 cells and induces apoptosis in HCT116 and SW480 cells in a concentration-dependent manner. 4beta-Hydroxywithanolide E (10 mg/kg per day for 14 days) reduces tumor growth in an HCT116 mouse xenograft model.Formal Name: 5beta,6beta-epoxy-4beta,14,17alpha,20,22R-pentahydroxy-1-oxo-ergosta-2,24-dien-26-oic acid, ?-lactone. CAS Number: 54334-04-2. Synonyms: NSC 212509. Molecular Formula: C28H38O8. Formula Weight: 502.6. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: Chloroform: soluble, Ethanol: soluble, Methanol: soluble. lambdamax: 217 nm. SMILES: O=C1[C@@]2(C)[C@@]([C@H]3C[C@]4([H])[C@]2([H])CC[C@@]5(C)[C@@]4(O)CC[C@@]5(O)[C@@](C)(O)[C@@]6([H])CC(C)=C(C)C(O6)=O)(O3)[C@@H](O)C=C1. InChi Code: InChI=1S/C28H38O8/c1-14-12-20(35-22(31)15(14)2)25(5,32)27(34)11-10-26(33)17-13-21-28(36-21)19(30)7-6-18(29)24(28,4)16(17)8-9-23(26,27)3/h6-7,16-17,19-21,30,32-34H,8-13H2,1-5H3/t16-,17+,19-,20+,21+,23-,24-,25-,26+,27-,28+/m0/s1. InChi Key: UPBUGICUKQIKTJ-KABTZXSUSA-N. Origin: Plant/Physalis peruviana.