4-Methylumbelliferyl palmitate

4-Methylumbelliferyl palmitate (4-MUP) is a fluorogenic substrate for lysosomal acid lypase (LAL). 4-MUP is cleaved by LAL (or by other acid lipases) to release the fluorescent moiety 4-MU. Recent advances allow the assessment of LAL activity in very small blood volumes using 4-MUP. Cholesterol ester storage disease and Wolman disease are recessive autosomal disorders caused by a deficiency in lysosomal acid lipase (LAL), also known as cholesteryl ester hydrolase. Spectral data: 4-MU fluorescence is pH-dependent. lambdaex 320nm (pH 1.97-6.72) and 360nm (pH 7.12-10.3), lambdaem 445-455nM (increasing as pH decreases). References: (1) G.J. Guy & J. Butterworth, Clin. Chim. Acta 84, 361 (1978) , (2) S. Kelly & R. Bakhru-Kishore, Clin. Chim. Acta 97, 239 (1979) , (3) T.G. Warner, et al., Am. J. Hum. Gen. 32, 869 (1980) , (4) T.G. Warner, et al., J. Biol. Chem. 256, 2952 (1981) , (5) M.W. Baillargeon & S.G. McCarthy, Lipids 26, 831 (1991) , (6) J. Hamilton, et al., Clin. Chim. Acta 413, 1207 (2012) , (7) H. Zhi, et al., J. Spectrosc. 1, 147128 (2013) , (8) T. Dairaku, et al., Mol. Gen. Metab. 111, 193 (2014) Appearance: White to off-white crystalline powder. Solubility: Soluble in DCM (10mg/ml), chloroform (20mg/ml) or DMF (10mg/m). Identity: Determined by 1H-NMR. Declaration: Manufactured by Chemodex. Inchi Key: QKQVFFACWLFRRX-UHFFFAOYSA-N Smiles: O=C1OC2=CC(OC(CCCCCCCCCCCCCCC)=O)=CC=C2C(C)=C1