4-Methylumbelliferyl-beta-D-Galactoside

Description: 4-Methylumbelliferyl-beta-D-galactoside (MUG) is a fluorogenic substrate for beta-galactosidase (beta-gal).1Hydrolysis of MUG by beta-gal releases the fluorescent moiety 4-MU, which displays a pH-dependent excitation maximum of 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively, and an emission maximum between 445-454 nm.2MUG has been used to detect beta-gal activity in intact bacteria, yeast, and mammalian cells.1. Smiles: Cc1cc(=O)oc2cc(O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)ccc12. References: Vidal-Aroca, F., Giannattasio, M., Brunelli, E., et al.One-step high-throughput assay for quantitative detection of beta-galactosidase activity in intact Gram-negative bacteria, yeast, and mammalian cellsBiotechniques40(4)433-434(2006)