3,6-diacetoxy Phthalonitrile

3,6-diacetoxy Phthalonitrile is a fluorescent probe for monitoring intracellular pH by flow cytometry in living cells. It rapidly crosses cell membranes and is cleaved by cytosolic esterases to form the fluorescent pH indicator 2,3-dicyano-hydroquinone (DCH), which is excited at UV wavelengths. DCH can be excited at 351 nm, with pH-dependent emission in the range of 450-476 nm (pH 5.0-10.0) using a potassium-based buffer.Formal Name: 3,6-bis(acetyloxy)-1,2-benzenedicarbonitrile. CAS Number: 83619-73-2. Synonyms: ADB, 1,4-Diacetoxy-2,3-dicyanobenzene, 2,3-Dicyano-1,4-hydroquinone diacetate. Molecular Formula: C12H8N2O4. Formula Weight: 244.2. Purity: >98%. Emission: 450-476 nm (pH 5.0-10.0). Excitation: 351 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS(pH7.2) (1:1): 0.5 mg/ml. lambdamax: 210, 305 nm. SMILES: CC(OC1=CC=C(OC(C)=O)C(C#N)=C1C#N)=O. InChi Code: InChI=1S/C12H8N2O4/c1-7(15)17-11-3-4-12(18-8(2)16)10(6-14)9(11)5-13/h3-4H,1-2H3. InChi Key: VXDCGBVAZDYTFK-UHFFFAOYSA-N.