Antibiotic UCN-01 (7-Hydroxystaurosporine, UCN-01)

Isolated from Streptomyces sp., Antibiotic UCN-01 inhibits protein kinase C (PKC) and cyclin-dependent kinase 2 (CDK2) resulting in accumulation of cells in the G1 phase and induction of apoptosis. UCN-01 also enhances the cytotoxicity of other anti-cancer drugs, such as DNA-damaging agents and anti-metabolite drugs, through putative abrogation of G2 and/or S phase accumulation induced by these anti-cancer agents. Solubility: Soluble in ethyl ethanol, DMF or DMSO. , Storage and Stability:, Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light.