WM3682 Viable Cells

Cells are sterile, validated by short tandem repeat profiling, and are tested as negative for mycoplasma. It is recommended that cell lines are tested for mycoplasma contamination and short tandem repeat (STR) profiling every 10 passages or each time a frozen seed stock is made. See cell culture protocol for additional details. WM3682 is a metastatic human melanoma cell line. This cell line was established from a lymph node metastasis site of a patient. This cell line contains a Q61L mutation at position 61 in the N-RAS gene. The Q61L mutation results in an amino acid substitution at position 61 in NRAS, from a glutamine (Q) to a leucine (L). WM3682 cells match with WM3702 and WM3758. These cells produce xenograft tumors when injected into immunocompromised mice.