SVG p12 Cells

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details). Description: SVG p12 is a human fetal glial cell line originally derived from fetal brain tissue and immortalized via transformation with SV40 large T antigen. It has been widely used as a model for studying neurotropic polyomaviruses, particularly JC polyomavirus (JCPyV), due to its glial origin and high permissiveness for viral infection. SVG p12 retains characteristics of astrocytic lineage and supports productive infection and propagation of JCPyV, making it a standard in vitro system for studying viral tropism, replication, and pathogenesis in glial cells. However, subsequent analysis has revealed that SVG p12 was contaminated with BK polyomavirus (BKPyV) after being deposited into cell repositories. Detection of BKPyV DNA and infectious virus in SVG p12 lines acquired from some culture collections has raised concerns regarding the integrity of experimental data derived from these cells. The contamination does not extend to all SVG-derived lines, as clones such as SVG-A have tested negative for BKPyV, suggesting that the contamination occurred during handling or distribution, rather than during the original derivation of the cell line. Due to its established use and robust responsiveness to polyomavirus infection, SVG p12 remains a key tool in virology research, particularly in the context of human neurovirology. Nevertheless, it is now recommended that researchers using this cell line verify the absence of BKPyV contamination in their stocks to ensure experimental reproducibility and data reliability. Organism Human Tissue Fetal brain Synonyms SVGp12, SVG(P12) Characteristics Age 8-12 fetus week Gender Male Ethnicity Unspecified Morphology Fibroblast Cell type Astrocyte Growth properties Adherent Regulatory Data Citation SVG p12 (Cytion catalog number 305878) Biosafety level 2 NCBI_TaxID 9606 CellosaurusAccession CVCL_3797 GMO Status GMO-S1: This human fetal glial cell line (SVG p12) contains SV40 Large T-Antigen sequences with an ori mutation and is additionally contaminated with BK polyomavirus strain UT, without deliberate genetic engineering of the contaminant. The SV40 insert is stably integrated. This classification applies only within Germany and may differ elsewhere. Biomolecular Data Mutational profile Handling Culture Medium EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a) Supplements Supplement the medium with 10% FBS Dissociation Reagent Accutase Fluid renewal 2 to 3 times per week Freeze medium As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing and Culturing Cells Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Incubation Atmosphere 37°C, 5% CO2, humidified atmosphere. Shipping Conditions Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately -78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage. Storage Conditions For long-term preservation, place vials in vapor-phase liquid nitrogen at about -150 to -196 °C. Storage at -80 °C is acceptable only as a short interim step before transfer to liquid nitrogen. Quality Control & Molecular Analysis Sterility Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.