SNU-668 Cells

Categories: Gastric cancer cell lines Description: The SNU-668 cell line is a human gastric carcinoma model originally derived from the poorly differentiated adenocarcinoma tissue of the stomach. This cell line has been widely used in studies of gastric cancer pathogenesis, signaling mechanisms, and drug responsiveness. Genomic characterization reveals that SNU-668 carries frequent mutations and chromosomal aberrations commonly observed in diffuse-type gastric cancers. Notably, it shows alterations in key oncogenic pathways such as TP53 mutation and possible activation of PI3K/AKT signaling, which may contribute to its tumorigenic properties and therapy resistance. SNU-668 has also been included in comprehensive multi-omics profiling projects such as the Cancer Cell Line Encyclopedia (CCLE), where it was assessed for transcriptomic, genomic, methylation, and proteomic signatures. The cell line exhibits distinct DNA methylation patterns and global histone modification profiles, which may play roles in epigenetic regulation of gene expression. Additionally, analysis of dependency maps has suggested lineage-specific vulnerabilities that could inform targeted therapy strategies for diffuse gastric carcinomas. As a model for stomach cancer with Asian ethnic background origin, SNU-668 continues to be an important tool in the preclinical evaluation of molecularly guided therapeutics. Organism: Human Tissue: Gastric Disease: signet ring cell adenocarcinoma Metastatic Site: Ascites Synonyms: SNU668, NCI-SNU-668 Age: 63 years Gender: Male Ethnicity: Korean Morphology: epithelial-like Cell Type: epithelial Growth Properties: Adherent, monolayer Citation: SNU-668 (Cytion catalog number 305635) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_5081 Mutational Profile: Mutation: KRAS, Simple, p.Gln61Lys (c.181C>A), Homozygous, Mutation: TP53, Simple, p.Ser215Asn (c.644G>A), Homozygous Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% heat inactivated FBS Dissociation Reagent: Accutase Doubling Time: 26 hours Subculturing: Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask Fluid Renewal: 2 to 3 times per week Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0