SCLC-21H Cells

Categories: Lung cancer cell lines Description: The SCLC-21H cell line was derived from the pleural effusion of a patient with small cell lung cancer (SCLC) of the oat cell subtype. This cell line, along with SCLC-22H, was established during a period of chemotherapy, with SCLC-21H being the second to be derived after an additional 15 days of treatment. While both cell lines originated from the same patient, they display significantly different biochemical, morphological, and kinetic properties. SCLC-21H, for example, has a faster population doubling time and a higher colony-forming efficiency compared to SCLC-22H. These differences make SCLC-21H a distinct tool for studying certain variant forms of SCLC. Biochemically, SCLC-21H differs from SCLC-22H in its low or undetectable levels of key neuroendocrine markers such as L-Dopa decarboxylase, bombesin, and carcinoembryonic antigen. However, both cell lines express high levels of neuron-specific enolase and creatine kinase isoenzyme BB, which are characteristic markers of SCLC. Moreover, while both cell lines exhibit c-myc amplification, SCLC-21H contains an additional rearranged and amplified EcoRI c-myc fragment, further highlighting its genetic uniqueness. Structurally, SCLC-21H exhibits loose growth in culture and features prominent nucleoli and abundant cytoplasm, contrasting with the more tightly packed morphology of SCLC-22H. The presence of ultrastructurally dense core granules in SCLC-21H confirms its neuroendocrine origin, and it is classified as representing a variant form of SCLC. These distinct features make SCLC-21H a valuable model for exploring the variant forms of small cell lung cancer and understanding their response to chemotherapy. Organism: Human Tissue: Lung Disease: Carcinoma Metastatic Site: Pleural effusion Synonyms: SCLC21H Age: 46 years Gender: Male Ethnicity: Caucasian Growth Properties: Suspension Citation: SCLC-21H (Cytion catalog number 300225) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0024 Oncogenes: myc amplification present, c-myc expression high Tumorigenic: Yes in nude mice Ploidy Status: Aneuploid Karyotype: Modal chromosome number 42/43, range 39-44. Chromosome deletion 3p. Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% heat-inactivatedFBS Dissociation Reagent: Accutase Doubling Time: 45 hours Subculturing: Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Suculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate for 10minutes at 37 degree Celsius. Collect the cells following centrigation, resuspend in fresh cell culture medium and count. Seeding Density: 2 to 4 x 104 cells/cm2 Fluid Renewal: 2 to 3 times per week Post Thaw Recovery: Cells will recover from freezing within 24 to 48 hours. Freeze Medium: As a cryopreservation medium, use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0