PDE7A-HEK293, rat recombinant cell line, 1x10(6) cells

Recombinant HEK293 cell line expressing rat PDE7A (phosphodiesterase 7A, accession number NM_031080). Each vial contains 1 X 10(6) cells in 1 ml of 10% DMSO. Functional validation: N-terminal FLAG-tagged rat PDE7A has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by rat PDE7A in rat PDE7A stably-expressed HEK293cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of luciferase reporter whereas rat PDE7A-HEK293 cells showed reduction in the level of cAMP, resulting in lowered levels of luciferase expression. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity. Storage: Store cells in liquid nitrogen upon arrival. Avoid freeze/thaw cycles. References: Malik, R. et al. (2008) Appl. Microbiol. Biotechnol. 77 (5): 1167-1173. Fan Chung, K. (2006). Eur. J. Pharmacol. 533(1-3):110-117.