NFAT Luciferase Reporter RAW264.7 Cell Line

The NFAT Luciferase Reporter RAW 264.7 Cell Line is a murine cell line that expresses the stably integrated firefly luciferase reporter under the transcriptional control of NFAT (Nuclear Factor of Activated T-cells)-responsive elements. This cell line has been validated to respond to mouse RANKL, Calcimycin (A23187) and PMA. It was also tested with cyclosporin A after activation with mouse RANKL or Calcimycin (A23187) and PMA.Interested in screening and profiling inhibitors, blocking antibodies, or activators of the NFAT pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening. Nuclear Factor of Activated T cells (NFAT) is a family of 5 transcription factors of near ubiquitous expression, known to have a central function in the immune system, for example by inducing the expression of various cytokines (such as IL-2, IL-3, IL-4, and TNFalpha (tumor necrosis factor alpha)) in T cells. NFATs cooperate with multiple other proteins to regulate distinct gene expression programs that determine the fate and function of T cell populations. The NFAT family also plays important roles in the nervous system, in the heart, and in skeletal muscles. In resting T cells, the NFAT protein is phosphorylated and confined to the cytoplasm in an inactive state. In response to a stimulus, an influx of calcium activates the Ca2+/calmodulin-dependent serine phosphatase calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins. This results in a conformational change that exposes a nuclear localization signal, promoting NFAT translocation to the nucleus. In the nucleus, NFAT proteins cooperate with other transcriptional regulators to induce gene expression. Through their role in the immune system, NFATs are involved in inflammation and these transcription factors are considered promising therapeutic targets in a variety of immune-related diseases. RAW 264.7 is a murine macrophage cell line that was transformed by the Abelson murine leukemia virus (A-MuLV) and is useful for studies involving immunoreactivity. The use of a luciferase reporter allows for easy readouts in cellular assays.